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Expand the Z-Stack control window. Highlight a channel that exhibits signal throughout the desired sample volume, then click the “Live” button. Use the focus knob to locate one end of the specimen, then click the Set First button. Focus to another end of the specimen, then click the Set Last...
Z-Stack Use this module to configure the acquisition of image Z stacks. The software controls the Z movement of the microscope at the correct intervals and synchronizes its movements with image acquisition. Step sizes can be computed automatically or determined interactively. If you want to check the settings made before...
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Z-Stacks 1. To make a z-stack first click on Z-Stack, a Z-Stack box should appear on the right if it doesn’t scroll down on the left until you see it. 2. If you would like to move the Z-stack box, …
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